SPIRILLUM CHOLERZ ASIATIC. 571 
in the zone of liquefaction. (See Figs 76 and 77.) 
Examined under a low-power lens, at the end of sixteen 
to twenty-four hours, the colonies appear as small, light 
yellow, round, coarsely granular disks, with a more or 
less irregular outline. In many cases at this stage an 
Fia. 76, Fia. 77. 
Cholera colonies in gelatin ; 
Cholera colonies in gelatin; twenty-four thirty-six to forty-eight hours’ 
hours’ growth. (DUNHAM.) growth. X about 30 diameters. 
ill-defined halo is seen to surround the granular colony, 
which by transmitted light has a peculiar reddish tint. 
The older the colonies become the more the granular 
structure increases, until a stage is reached when the 
surface looks as if it were covered with little fragments 
of broken glass (Koch). Liquefaction continues around 
the colonies, their structure appears fissured and coarsely 
granular in texture, and occasionally a hair-like border 
is formed at the periphery (Fig. 78), or a gray trans- 
parent zone, until the entire colony breaks up into frag- 
ments. Sometimes the colonies may be retained as com- 
pact masses in the zone of liquefaction, and then they are 
