SPIRILLUM CHOLER ASIATIC. 573 
bator. In bouillon the growth is rapid and abun- 
dant; in the incubator at the end of ten to sixteen hours 
the liquid is diffusely clouded, and on the surface a 
wrinkled membranous layer is often formed. In gen- 
Fic. 79. 
oT 
A characteristic series of cholera cultures in gelatin; one, two, three, four, 
and six days’ growth. (DUNHAM.) 
eral the spirillum grows in any liquid containing a 
small quantity of organic matter and having a slightly 
alkaline reaction. An acid reaction of the culture me- 
dium prevents its development, asa rule; but it has the 
power of gradually accommodating itself to the pres- 
ence of vegetable acids, and grows upon potatoes, in 
the incubator only, which have a slight acid reaction. 
Abundant development occurs in bouillon which has 
been diluted with eight to ten parts of water and in 
