592 BACTERIOLOGY. 
as since its discoverey, though repeatedly sought for, it 
has seldom been found by subsequent investigators. 
In 1884, Miller observed a curved bacillus in a hollow 
tooth, which from its behavior in microscopical prepa- 
rations, in cultures and animal experiments, is probably 
identical with the Finkler and Prior spirillum; and 
other very similar spirilla have been found by others. 
DENEKE’S CHEESE SPIRILLUM. 
Obtained by Deneke (1885) from old cheese, but since 
then rarely met with. Morphologically and culturally 
it shows greater similarity to Koch’s comma bacillus 
than the Finkler and Prior spirillum, but can be read- 
ily differentiated from it also. 
Morphology. Curved rods and long spiral filaments, 
somewhat more slender than the cholera spirillum, the 
turns in the spiral threads being lower and closer 
together. Has a single flagellum attached to one end. 
Stains with the usual aniline colors. 
Biological Characters. An aérobic and facultative 
anaérobiec, liquefying, motile spirillum. Does not form 
spores. Upon gelatin plates small, punctiform colonies 
are formed at the end of twenty-four hours, which when 
slightly magnified are seen to be circular in shape, with 
sharply defined border and of a greenish-brown color 
in the centre and paler toward the margins. Later, 
when liquefaction has commenced, the sharp contour is 
often lost. The liquefaction progresses more rapidly 
than with the cholera bacillus, but not so energetically 
as with the spirillum of Finkler and Prior. In gelatin 
stick cultures after forty-eight hours a stocking-like 
pouch is developed, the spirilla sinking to the bottom 
