CHAPTER XXXY. 
GLANDERS BACILLUS. 
BACILLUS MALLEI (Bacillus of Glanders). 
Tus bacillus was discovered and proved to be the 
cause of glanders by isolation in pure culture and com- 
munication to animals by inoculation, by several bacte- 
riologists almost at the same time (1882), viz., by the 
investigations of Léfiler, Schiitz, Israel, Bouchard, 
Charrin, Weichselbaum, Kauzfeld, and Kitt. It is 
found in the recent nodules in animals affected with 
glanders, and in the discharge from the nostrils, pus- 
from the specific ulcers, etc., and occasionally in the 
blood. . 
Morphology. Small bacilli with rounded or pointed 
ends, from 0.25 to 0.4 broad and from 1.54 to 3n 
long; usually single, but sometimes united in pairs, or 
growing out to long filaments, especially in potato cul- 
tures. Frequently breaks up into short, almost coccus- 
like elements (Fig. 81). 
The bacillus mallei stains with difficulty with the 
aniline colors, best when the aqueous solutions of these 
dyes are made feebly alkaline; it is decolorized by 
Gram’s method. This bacillus presents the peculiarity 
of losing very quickly in decolorizing solutions the color 
imparted to it by the aniline staining solutions. For 
this reason it is difficult to stain in sections. Léffler 
recommends his alkaline methylene-blue solution for 
