GLANDERS BACILLUS. 599 
staining sections, and for decolorizing a mixture con- 
taining 10 c.c. of distilled water, 2 drops of strong 
sulphuric acid, and 1 drop of a 5 per cent. solution of 
oxalic acid; thin sections to be left in this acid solution 
for five seconds. 
Fie, 81. 
Glanders bacilli. Agar culture. x 1000 diameters, 
Biological Characters. An aérobic, non-motile bacil- 
lus, whose molecular movements are so active that they 
have often been taken for motility. It grows on vari- 
ous culture media at 37° C. Development takes place 
slowly at 22° C. and ceases at 43° C. It does not form 
spores. Exposure for ten minutes to a temperature of 
55° C., or for five minutes to a 3 to 5 per cent. solution 
of carbolic acid, or for two minutes to a 1: 5000 solu- 
tion of mercuric chloride, was effectual in destroying 
its vitality. Asa rule, the bacilli do not grow after 
having been preserved in a desiccated condition for a 
week or two; in distilled water they are also quickly 
destroyed. The bacillus does not grow in infusions of 
