GLANDERS BACILLUS. 601 
is often lobulated; other lymphatic glands become in- 
flamed, and some of them suppurate and open exter- 
nally, leaving deep, open ulcers; the lungs are also 
involved, and the breathing becomes rapid and irreg- 
ular. In farey, which is a more chronic form of the 
disease, circumscribed swellings, varying in size from 
a pea to a hazel-nut, appear on different parts of the 
body, especially where the skin is thinnest; these sup- 
purate and leave angry-looking ulcers with ragged 
edges, from which there is an abundant purulent dis- 
charge. The bacillus of glanders can easily be obtained 
in pure cultures from the interior of suppurating nod- 
ules and glands which have not yet opened to the sur- 
face, and the same material will give successful results 
when inoculated into susceptible animals; but the dis- 
charge from the nostrils or from an open ulcer contains 
comparatively few bacilli, and these being associated 
with other bacteria which grow more readily on the 
culture media than the bacillus mallei, it is not easy to 
obtain pure cultures by the plate method from such 
material, and here animals are useful. 
Of test animals guinea-pigs and field-mice are the 
most susceptible. In guinea-pigs subcutaneous injec- 
tions are followed in four or five days by swelling at 
the point of inoculation, and a tumor with caseous con- 
tents soon develops; then ulceration of the skin takes 
place, and a chronic purulent ulcer is formed. The 
lymphatic glands become inflamed and general symp- 
toms of infection are developed in from two to four 
weeks; the glands suppurate and in males the testicles 
are involved; finally purulent inflammation of the joints 
occur, and death ensues from exhaustion. The forma- 
tion of the specific ulcers upon the nasal mucous mem- 
