GLANDERS BACILLUS. 605 
eighteen hours after the injection. This elevation of 
temperature is from 1.5° to 2° C.,, or even 4° C., above 
the normal mean temperature. Ina healthy animal the 
rise of temperature, as a rule, amounts to only a few 
tenths of a degree, but it may reach 1° C. The rise of 
temperature, however, should be considered always in 
connection with the general and local reactions. Ina 
glanderous animal, after an injection of mallein, the 
general condition is more or less profoundly modified. 
The animal has a dejected appearance; the countenance 
is pinched and anxious, the hair is rough, the flank is 
retracted, the respirations are rapid, there are often 
rigors, and the appetite is gone. In healthy animals 
the general symptoms do not occur. The local reaction 
around the point of injection in a glanderous animal is 
usually very marked. A few hours after the injection 
there appears a large, warm, tense, and very painful 
swelling, and running from this will be seen hot, sen- 
sitive lines of sinuous lymphatics, directed toward the 
neighboring lymphatic nodes. This cedema increases 
for twenty-four to thirty-six hours and persists for 
several days, not disappearing entirely for eight or ten 
days. In healthy animals, at the point of injection 
mallein produces only a small oedematous tumor, and 
the cedema, instead of increasing, diminishes rapidly and 
disappears within twenty-four hours. The value of this 
test has been demonstrated by numerous experiments. 
There are some exceptions to the rule as described 
above, but they are infrequent, and mallein has been 
used with considerable success as a diagnostic aid in 
detecting the existence or absence of glanders in doubt- 
ful or obscure cases. 
