628 APPENDIX. 
dry specimens these bodies stain deeply with methylene- 
blue, and they are solid or vesicular in form. If the 
examination be made within twelve to eighteen hours 
after the chill the hyaline bodies are seen to have 
grown to occupy one-fourth to one-third of the bodies 
of the red cells. They are more pigmented, and the 
corpuscles containing them have become gradually paler 
and somewhat expanded. The pigment granules, which 
at first are small, increase in size, and the organisms 
show very active ameeboid movements. At the end of 
forty-eight hours they occupy entire corpuscles, are very 
sluggish in their movements, and look like thin, trans- 
lucent shells, and are usually devoid of color. Many 
of the organisms then undergo the remarkable change 
known as segmentation, which precedes and is asso- 
ciated with chills and fever. The amceboid movement 
ceases as well as that of the pigment granules. The 
latter gradually collect toward the centres of the ameebee 
until they are in the form of closely packed, more or less 
central clumps. The protoplasm becomes more finely 
granular, and indistinct lines of striation are seen, which 
begin at the periphery. At this stage the organisms 
may present the appearance of rosettes. The segmen- 
tation progresses until the entire protoplasm is divided 
into twelve to eighteen or twenty spheres. The shell 
of the corpuscles containing a parasite usually bursts, 
and the small, rounded, hyaline bodies are set free. 
Each one of these little bodies consists of a translucent 
protoplasm, with a central, more highly refractile spot. 
In stained preparations, during the segmenting process, 
the reticulum becomes denser and sharper, and then 
breaks up into fifteen to twenty small spheroidal 
spores. 
