AM@BA COLI. 645 
amcebe, the inoculations have been made in three 
ways: by feeding animals with material containing the 
amceba, by inoculation of the small intestine after a 
preliminary laparotomy, and, finally, by rectal injec- 
tions with or without suture of the anal orifice. The 
first method has always proved unsuccessful except 
when encysted forms were present. To the second 
method the objection has been raised that the manipu- 
lation of the intestines and the use of antiseptic solu- 
tions during the course of the operation are in them- 
selves a source of irritation to the bowel, and in some 
cases have produced an enteritis. The third method 
is the simplest, and has given positive results in the 
hands of Lisch, Kruse, Pasquale and others. 
The results of the last two observers were as fol- 
lows: Dysenteric stools, or material from hepatic 
abscesses containing amcebe, were injected into the 
rectum of various animals, with or without subsequent 
closure of the anus, for twenty-four or forty-eight 
hours. In some cases, chiefly those in which motion- 
less amcebee were injected, no abnormal result followed; 
in others, blood-tinged mucus, containing actively 
moving amcebe, appeared in the evacuations from the 
second day or thereabouts, but the animals did not 
appear to be ill; in a third series, with evacuations of 
a like character, the animals wasted and died after 
a variable number of days. In both the second and 
third series of cases post-mortem examination showed 
pathological changes in the large intestine, proportion- 
ate, as a rule, to the severity of the symptoms. Of 
especial interest are the experiments made with material 
from liver abscesses which were proved to contain no 
other organism than the ameba. Three such cases are 
