658 APPENDIX. 
the calves and the trial vaccinations in young children 
give a much more’ reliable basis for judging of the 
character of the virus than any bacteriological counts 
.of colonies. 
Pathogenic bacteria other than the practically non- 
_virulent skin staphylococci are not found when animals 
are properly kept and vaccinated. The vaccine pulp 
and serum mixture is added to two and one-half to 
three and one-half times its bulk of a mixture consisting 
of two parts of chemically pure glycerin and one part 
of water. 
Efficient vaccine should be inoculated in a portion of 
skin no more than one-eighth inch in diameter. 
Care of the Calves. All bedding is avoided and an 
exclusively milk diet given; thus much of the otherwise 
unavoidable dust is done away with. 
