ao MICEO-ANALYTICAL METHODS 



Form No. Ill 

 Bacteriological Examination 



(No., label, dates, condition of seals as for form I.) 



I. Direct count. (Thoma-Zeiss hemacytometer with Turck ruling.) 



1 . Bacilli per cc 



2. Cocci per cc 



II. Plate and tube cultures. (Lactose-litmus-agar.) 



1. Temperature differential test. 



a. (20° C.) colonies per cc 



b. (38° C.) colonies per cc 



2. Color differential test. 



a. Pink or yellow colonies per cc 



b. Not pink or yellow colonies per cc 



3. Gelatin liquefying colonies per cc 



4 . Indol reaction ( ± ) 



5. Neutral red reduction ( + ) 



6. Gas (hydrogen) formula 



7. Gram stain behavior ( + ) 



8. Presumptive colon bacillus test ( + ). 



a. Amounts used 



b. Number of tests 



c. Rating 



III. Special tests 



IV. Conclusions. 



. Analyst. 



