TABLE OF CONTENTS. 



List of Apparatus X 



Laboratory Rules XI 



List of References XII 



PART I.— GENERAL BACTERIOLOGY 

 Chaptee I. Morphology and Elementary Technique. 



3. 



4. 

 5. 



6. 



7. 



8. 



9. 



10. 



11. 

 12. 

 13. 



14. 

 15. 



16. 



17. 

 18. 



10 



EXERCISE P 



1. Cleaning Glassware 



2. Plugging Flasks and 



Tubes ■ 



Sterilization of Glassware 

 Preparation of Bouillon. . . 

 Filling Test-tubes and 

 Flasks with Culture Me- 

 dia 



Sterilization of Culture 



Media 10 



Preparation of Gelatin ... 14 



Preparation of Agar 16 



Preparation of Potatoes.. 18 

 Preparation of Water- 



blanks 18 



Care of Culture Media 18 



Platinum Needles 20 



Test-tube Cultures 22 



Incubation of Cultures. ... 24 

 Study of Test-tube Cul- 

 tures 26 



Cleaning Slides and Cover- 

 glasses 26 



Preparation of Staining 



Solution 28 



Simple Cover-glass Prep- 

 arations 30 



EXERCISE PAGE 



Use of Microscope 34 



19. 

 20. 

 21. 



22. 



23. 

 24. 

 25. 

 26. 

 27. 

 28. 



29. 



30. 

 31. 

 32. 

 33. 

 34. 



35. 

 36. 



36 



38 



40 

 42 

 46 



Drawing Bacteria 



Hanging-drop Prepara- 



tion 



Microscopical Study of 



Form Types 



Study of Cell Grouping. . . 

 Study of Involution Forms 



Study of Endospores 46 



Flagella Stain 48 



Capsule Stain 50 



Stain for Metachromatic 



Granules 50 



Morphology of Yeasts and 



Moulds Compared with 



Bacteria 50 



Gelatin Plate Cultures. ... 52 



Agar Plate Cultures 56 



Roll Cultures 56 



Study of Plate Cultures.. . 58 

 Use of Decolorizing 



Agents 60 



Gram 's Stain 60 



Tubercle Stain (Gabbett) 62 



Chapter II. Physiology op Bacteria. 



EXERCISE PAGE 



37. Preparation of Special 



Media 64 



38. Effect of Reaction of Me- 



dia on Growth 66 



39. Effect of Concentration of 



Media on Growth 66 



(VII) 



EXERCISE PAGE 



40. Effect of Temperature 

 Variations on Rate of 

 Growth 68 



41. Determination of Thermal 

 Death Point 68 



