64 GENERAL BACTERIOLOGY 



CHAPTER II 

 PHYSIOLOGY OF BACTERIA 



EXERCISE 37. PREPARATION OF SPECIAL MEDIA. 



The following media will be necessary for the work outlined in 

 this chapter : 



a. Dextrose Bouillon. To ordinary bouillon add 1% dextrose 

 (c. p.), tube and sterilize in steamer, not in autoclave, 7 test-tubes 

 and 2 fermentation tubes. 



6. Dextrose Gelatin. 1% dextrose (c. f.), tube and sterilize 

 in steamer, 6 tubes. 



c. Dextrose Agar. 1% dextrose (c. p.), tube and sterilize in 

 steamer, 5 tubes. 



d. Lactose Agar. 1% lactose (c. p.), tube and sterilize in 

 steamer, 2 tubes. 



e. Litmus Solution. To 10 gms. of the dried material add 500 

 cc. of distilled water, digest in a warm place, decant clear liquid and 

 add a few drops of nitric acid to produce a violet color. (Sutton.) 

 Place in flasks or test-tubes and sterilize in steamer three times, 1 

 tube. 



/. Dexteose-free Broth. This is prepared from beef by inocu- 

 lating the meat infusion with an organism capable of fermenting 

 sugar, such as B. coli, and allowing it to stand several hours at 38° C. 

 (Between b. and c. Exercise 4.) The bouillon is then prepared in 

 the usual manner.^ 

 Or Dunpiam's Solution. 



Sodium chloride 0.5 gm. ) . n • j- i ■, ^i. 



T, . /iTT-.^ X 1 Isoil until all IS dissolved, filter, 



Peptone (Witte) 1. gm. V- ^ ^. -t , ^ \ 



„r i -1 nn tube and sterilize, 4 tubes. 



Water 100. gms. ) ' 



g. Nitrate Solution. 



I Smith: Jour. Exp. Med., 1897, 3: 543. 



