98 Physiological Characters 



1. Relation to temperatuee: 



optimum °C. ; limits to °C.; 



thermal death-point °C ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Kblation to other aqents, such as 



desiccation, light, disinfectants, etc.: — . 



i. Pigment production:. 



5- Gas production in sugar media: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm = . 



gas formula, H: CO2: : : 



b. lactose c. saccharose 



6. Aoid or ALKALI production: 



litmus milk 



7. Reduction op nitrates: .- 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatio 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) :. 



