102 Physiological Chaeacters 



1. Relation to temperature: 



optimum "" C. ; limits to °C. ; 



tliermal deatti-point °C.: time of exposure minutes; 



medium in wliich enposure is made 



2. Relation to free oxygen : 



3. Relation to other agents, such as 



desiccation, liglit, disinfectants, etc-: 



4. Pigment production:, 



GtAS production in sugar media: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H: CO2: : ; 



6. lactose c. saccharose 



Acid or alkali production: 



litmus milk 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



