106 Physiological Characters 



1, Relation to temperature : , 



optimum °C. ; limits to °C. ; 



tliermal death -point "C; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc-; 



I Pigment production: 



5. Gas production in sugar media: 



n. dextrose (1) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : : 



h, lactose e. saccharose .' 



6. Acid or alkali production : 



litmus milk 



7- Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Xndol production 



48 hours days 



9. En5;tmb production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odok: 



11. Pathogenesis (or other special characters) : 



