112 Physiological Chaeactees 



1. Relation to TEMPEKATnEE: 



optimum °0. ; limits to °C. ; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxtoen : 



3. Relation to other agents, such as — 

 desiccation, light, disinfectants, etc- : 



i. Pigment production:.. 



Gas production in sugar media: 



I*, dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



' rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent hours per cent, 



reaction in open arm 



gas formula, H: CO2: : 



b. lactose i^. saccharose. 



6. Acid or ALKALI production: 



litmus milk 



7. Reduction of nitrates: 



to nitrites to ammozna. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odoe: 



11. Pathogenesis (or other special characters) : 



