144 Physiological Characters 



1. Relation to temperature : 



optimum ° C. ; limits to °C 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxysen: 



3. Relation to other agents, such as — 

 desiccation, light, disinfectants, etc. : 



4. Pigment production:. 



6. G-AS production in sugar media: 



u. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : : 



b. lactose <;. saccharose 



6. Acid or alkali production: 



litmus milk 



7. Reduction op nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



