148 Physiological Characters 



1. Relation to tempeeatobb : 



optimum °C. ; limits to "C ; 



thermal death-point °C.; time of exposure minutes: 



medium in which exposure is made 



2. Relation to feee oxygen : 



3 Relation to other agents, such as 



desiccation, light, disinfectants, etc.:—. 



4. Pigment production: 



Gas production in sugar media: 



a, dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hoars per cent., 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gras formula, H: CO2: : 



b. lactose c. saccharose. 



Acid or alkali production: 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia.. . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Chakacteeistio odor: 



11. Pathogenesis (or other special characters) : 



