156 Physiological Characters 



1. Relation to temperature : 



optimum * C. ; limits to ''C. ; 



thermal death-point °C.; tim.eof exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



S. Relation to other agents, such as , 



desiccation, light, disinfectants, etc: — . 



4. Pigment pro'uuction:., 



Gas production in sugar media: 



f(. dextrose (1) Shake culture: 



(2J Ferm.entation tube, growth in open ^rm closed arm 



rate of development: 24 hoars per cent., 48 hours percent. 



72 hours per cent hours per cent 



reaction in open arm 



gas formula, H : CO2 : : 



h. lactose c. saccharose. 



Acid or alkali production : 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic ■. 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



