162 Physiological Characters 



1. Relation to temperature: ; 



optimum °C. ; limits to °C. ; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other asents, such as 



desiccation, light, disinfectants, etc :— . 



J. Pigment production: 



5. Gas PRODnCTiON in sugar media: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H : CO2 : : : , 



he lactose c. saccharose 



6. Acid or alkali production: 



litmus milk. 



7. Reduction oe nitrates: 



to nitrites to ammonia... 



8 Indol production 



48 hours .' days . 



9. Enzyme production: 



proteolytic ' 



digestion of gelatin digestion of casein 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



