166 Physiological Characters 



1. Relation to temperature: 



optimum °C.; limits to °C.; 



thermal death-point °C. ; time of exposure minutes ; 



medium in which exposure is made 



2. Relation to free oxygen : 



3. Relation to other agents, such as , 



desiccation, light, disinfectants, etc: — . 



L Pigment production:, 



Gas production in sugar media: 



«.. dextrose (1) Shake culture: 



(2J Fermentation tube, grrowth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours percent., hours percent. 



reaction in open arm 



gas formula, H: CO2: : , 



h. lactose u. saccharose. 



Acid or alkali production: 



litmus milk. 



7. Reduction of nitrates; 



to nitrites to ammonia... 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of erelatiu digestion of casein. 



diastatic 



10. Characteristic odor; 



11. Pathogenesis (or other special characters) : 



