170 Physiologipal Characters 



1. Relation to teuiperature : , , 



optimum °C. ; limits to °C. i 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation to peek oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc : 



4. Pigment production: 



Gas production in sugar media: 



It. dextrose (1) Shake culture: 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : ; 



b. lactose u. saccharose - 



Acid or alkali production: 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia.. . 



8. Indol production 



48 hours days . 



3. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



fO. Characteristic odob: 



11. Pathogenesis (or other special characters): 



