174 Physiological Characters 



1. Relation to temperature : 



optimum "C; limits to °C.; 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Kelation to free oxygen: 



3- Relation to other agents, such as 



desiccation, light, disinfectants, etc.: — . 



i. Pigment peoduotion:. 



Gas production in sugar media: 



a. dextrose (1) Shake culture : 



■ (2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



b. lactose u. saccharose. 



AOID or ALKALI production: 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia. ■ . 



8. INDOL PRODUCTION 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



