178 Physiological. Chaeacters 



1. Relation to tempeeatuee : 



optimum ° C. ; limits to °C. ; 



thermal death -point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. :- 



4. Pigment peoduction:, 



Gas production in sugae media: 



ct. dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



b. lactose c. saccharose . 



6. AOID OE ALKALI PKODUCTION : 



litmus milk 



7. Reduction of niteates: 



to nitrites to ammonia. . . 



8. Indol peoduction 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11 . Pathogenesis (or other special characters) : 



