190 Physiological Chaeactees 



1. Belation to tempebatube: 



optimum °C. ; limits to "C; 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation to feee oxysen: 



3- Relation to other agents, such as — 

 desiccation, ligrht, disinfectants, etc- : 



4. Pigment production:. 



Gas production in sugar media : 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm. . . .' 



gas formula, H: CO2: : ; 



b. lactose c. saccharose 



Acid or alkali production : 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



8. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Charaoteeistio odor: 



11. Pathogenesis (or other special characters) : 



