194 Physiological Chaeacteks 



Relation to tempeeatuke; , , 



optimum °C.; limits to °C.; 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



Relation to feee oxygen : 



3. Relation to other agents, such as 



de"!ieoation, light, disinfectants, etc. ; — . 



4. Pigment pkoduction;. 



6. Gas production in sugar media: 



14. dextrose ( 1 ) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 21 hours percent., 48 hours percent. 



72 hours percent., hours percent. 



reaction in open arm 



gas formula, H: CO2: : ; 



b. lactose c. saccharose 



6. Acid or alkali production : 



litmus milk ■ 



7. Reduction OF nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



