108 Phtsiological Characters 



1. Relation to temperature : 



optirmim °C.; limits to °C.; 



thermal death-point °C.; time of exposure .....minutes; 



medium in which exposure is made 



Z. Relation to free oxygen : 



3- Relation to other agents, such as — 

 desiccation, light, disinfectants, etc.: 



*- Pigment production;. 



Gas production in sugar media: 



a. dextrose (1) Shake culture: 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent, 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



&. lactose V. saccharose. 



Acid or alkali production : 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours day s . 



9. Enzyme production: 



proteolytic 



die:estion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



