210 Physiological Chaeacters 



1. Relation to temperature : , 



optimum "C. ; limits to °C. ; 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation TO FREE oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. ; 



4. Pigment production:., 



Gas production in sugar media: 



w. dextrose (1) Shalce culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H: COz: : 



h. .lactose t. saccharose. 



Acid or alkali production : 



litmus milk 



Reduction of nitrates: 



to nitrites to ammonia. . . 



Indol production 



48 hours days . 



Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



