222 Physiological Characters 



1. Relation TO temperature: 



optimum °C.;limits to °C.; 



thermal death-point °C.; time of exposure minutes;- 



medium in which exposure is made 



2. Relation to free oxygen : 



■5. Relation to other agents, such as 



desiccation, light, disinfectants, etc- :- 



4. Pigment production: 



Gas production in sugar media: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H; CO2: : ; 



b. lactose c. saccharose 



Acid or alkali production: 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia.. . 



8. InDOL PRODUCTION 



48 hours days . 



9. Enzyme PRODUCTION: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) :. 



