226 Physiological Charactees 



1. Relation to tempeeatuee : 



optimum °C.:limits ;to °C.; 



thermal death-point °C.; time of exposure minutes; 



medium in w-hich exposure is made 



2, Relation to free oxygen : 



3. Relation to othee agents, such as 



desiccation, light, disinfectants, etc- : 



i. Pigment pkoduotion: 



Gas peoduction in sugae media: 



a. dextrose (1) Shake culture: 



{2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



b, lactose c. saccharose. 



6. Acid OE ALKALI peoduction: 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia... 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Chaeacteeistio odor: 



11. ' Pathogenesis (or other special characters); 



