230 Physiological Characters 



1. Relation to temperature: 



optimum "C. ; limits to °C. ; 



thermal death-point °C. : time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen : 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. ; 



I. Pigment production;. 



5. GrAS PRODUCTION IN SUGAR MEDIA: 



u. dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 21 hours percent., 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H:C02: : ; 



6. lactose c. saccharose 



6. Acid or alkali production : 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



