334 Pl-IYSIOLOGIOAL CHARACTERS 



1. Relation to tempehaturb : 



optimum °C. : limits to *^C,; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. :- 



4. Pigment production: 



Gas production in sugar media: 



a. dextrose (1) Shake culture: 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



b. lactose c. saccharose. 



6. Acid OR ALKALI production: 



litmus milk 



7. Reduction op nitrates: 



to nitrites to ammonia... 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Chakactbristic odok: 



11. Pathogenesis (or other special characters) : . 



