238 Physiological Characters 



1. Relation to tempeeatuke : 



optimum ° C. ; limits to "C. ; 



thermal death-point °C. ; time of exposure minutes ; 



medium in which exposure is made 



2. Relation to free oxygen: 



3- Relation to other agents, such as.... 

 desiccation, light, disinfectants, etc. ; 



4. Pigment production:, 



Gas production in sugar media: 



M. dextrose (1) Shake culture: 



{2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours per cent. 



1 eaction in open arm 



gas formula, H: CO2: : ; 



h. lactose c. saccharose 



Acid or alkali production: 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) :. 



