342 Physiological Charactees 



1. Relation to temperature: 



optimum °C.;liinits to °C.; 



thermal death-point °C. ; time of exposure minutes ; 



medium in which exposure is made 



2. Relation to free oxygen : 



3- Relation to other agents, such as 



desiccation, light, disinfectants, etc- :- 



4. Pigment production: 



Gas production in sugar media: 



It. dextrose (1) Shake culture: 



(2J Fermentation tube, growth in open arm ." closed arm 



rate of development: 24 hoars per cent., 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H; CO2: : : 



6. lactose c. saccharose 



Acid or alkali production: 



litmus milk. 



7. Reduction op nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: , 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



