246 Physiological Characters 



1. Relation TO TEMPEKATUEE: 



optimum °C.;limits to °C.; 



thermal death-point "C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. ; 



4. Pigment pkoduction:, 



G-AS production in sugar media: 



ct. dextrose (1) Shake culture: 



(2) Fermentation tube, errowth in open arm closed arm 



rate of development: 24 hours percent.. 48 hours percent. 



72 hours per cent hours percent. 



reaction in open arm 



gras formula, H: CO2: : 



b, lactose v. saccharose. 



Acid ok alkali production: 



litmus milk 



7. Reduction of nitrates: 



to nitrites to ammonia.. . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



