250 Physiological Characters 



1. Relation to tempeeatube : 



optimum "0. ; limits to °C. ; 



thermal death-point °0._; time of exposure minutce; 



medium in which exposure is made 



2. Relation to free oxygex: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc : 



4. Pigment production: 



5. Gas production in sugar media: 



It. dextrose (1 ) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours percent, 



reaction in open arm 



gas formula, H: CO2: : 



h. lactose c. saccharose. 



AdiD or ALKALI production: 



litmus milk 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 b ours days . 



9. Enzyme production: 



proteolytic ■. 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odoe: 



11. Pathogenesis (or other special characters) :. 



