EXERCISE 92. CHOLERA GROUP. ggi 



Microspira comma (Koch) Schroeter. 



Synonyms. Spirillum cholerae-asiaticae Zopf; Vibrio choleras 

 Lehm. & Neum. ; Comma bacillus ; Cholera bacillus. 



Explanatory. First described by Koch in 1884. Found in 

 the intestinal contents of cholera patients. It has also been isolated 

 several times from water supplies. 



References. Koch, Berl. Klin. Wochenschr., 1884, no. 31 u. 32 ; 

 A. 446 ; C. 335 ; Fl. 2, 527 ; H. 333 ; K. & W. Ill, 1 ; L. & N. 353 ; Mig. 

 2, 960 ; M. & R. 407 ; M. & W. 152 ; McF. 442 ; P. 568. 



Morphological characters : 



1. Form and arrangement: 

 a. Bouillon 



Sketches. 



b. Agar . 



I. Gelatin 



d. Other media. , 



2. Size: 



3. Staining powers: 



a. Aqueous gentian- violet. . 



b. Loeffler's methylen-blue. 



c. Gram's stain 



d. Special stains 



4. Motility : 



a. Character of movement. . . 



b. Plagella stain 



5. Spokes : 



6. Special ohakacters: 



((. Capsules 



b. Involution forms 



v. Deposits or vaeuoles.. 

 iZ. Pleomorphism 



