254 Physiological Chaeacters 



1. Relation to tempekatuee : 



optimum °C.;limits to °C.; 



thermal death-point °C. ; time of exposure minutes ; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc- ; 



i. Pigment production: 



Gas production in sugar media : 



a. dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hour:5 percent. 



72 hours per cent.. hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



6. lactose u. saccharose. 



Acid or ALKALI production: 



litmus milk. 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



disesti on of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



