258 Physiological Characters 



1. Relation to temperature : 



optimum °C. ; limits to °C.; 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2, Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. — 



4. Pigment peoduction: 



5. Gas production in sugar media: 



a, dextrose (1) Shake culture: 



(2; Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H: CO2: : ; 



h. lactose c. saccharose 



6. Acid ok alkali production: 



litmus milk . 



7. Reduction OF NITRATES: 



to nitrites to ammonia. . . 



8. InDOL PRODUCTION 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



