362 Physiological Characters 



1. Relation to tempekature : 



optimum °C.;limits to °C.; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to fbee oxygen: 



3. Relation to other agents, such as — 

 desiccation, light, disinfectants, etc. ; 



4. Pigment production: 



6. Gas production in sugar media: 



«. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hoars per cent., 48 hours percenj. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : : 



h. lactose c. saccharose 



6. Acid ok alkali production: 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



