366 Physiological Characters 



1. Relation to tessperature : , 



optimum °C.: limits to °C.- 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc:—. 



4. Pigment production:. 



5. Gas production in sugar media: 



u. dextrose (1) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



&. lactose G. saccharose 



6. Acid or alkali production: 



litmus milk. 



7. Reduction OP nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



