270 Physiological Characters 



1. Kelation to temperature : 



optimum °C.;limits to °C.; 



thermal death-point "C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc :- 



4. Pigment production:.. 



5. Gas production in sugar media: 



». dextrose (1) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hoars per cent.. 48 hours ..percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



I. lactose V. saccharose. 



6. Acid ok alkali production: 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Chaeacteristio odor: 



11. Pathogenesis (or other special characters) : 



