276 Physiological Characters 



1, Relation TO temperatuke: 



optimum °C. : limits to. °C, ; 



thermal death-point °C.; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen:. 



3. Relation to other agents, such as 



desiccation, ligrht, disinfectants, etc-: — . 



i. Pigment production: 



5. Gas production in sugar media: 



It. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development; 24 hours percent.. 48 hours percent. 



72 hours per cent hours per c£nt. 



reaction in open arm 



eras formula, H; CO2: : 



6. lactose t. saccharose. 



Acid or alkali production: 



litmus milk. 



7. Reduction of nitrates; 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic ODOR: 



11. Pathogenesis (or other special characters) : 



