280 Physiological Chaeaotees 



1. Relation to temperature : 



optimum °C.;limits to °C.; 



thermal death-point °C.; time of exposure minutes: 



medium in which exposure is made 



2. Relation TO PREE oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc :— . 



4. Pigment production: 



5. Gas production in sugar media: 



It. dextrose (1) Shake culture: 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gras formula, H : CO2 : : ; 



6. lactose c. saccharose 



6. Acid or alkali PRODUCTION: 



litmus milk 



7. RbDUJTION OP NITRATES; 



to nitrites to ammonia. . . 



8. INDOL PRODUCTION 



48 hours days. 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



