288 Pl.TSICLOGICAL CHARACTERS 



1. Relation to temperature: , 



optimum °C.;limits to °C.; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation TO FREE oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc :— . 



4. Pigment production: 



Gas production i:t sugar media: 



a, dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours percent hours percent. 



reaction in open arm 



gas formula, H : CO2 : : : 



b. lactose c. saccharose 



Acid or alkali production : 



litmus milk 



7. Reduction of nitrates: 



to nitrites to ammonia.. . 



8. Indol production ." 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestit)n of casein. 



diastatie 



10. Characteristic odor: 



11. Pathogenesis (or other special characters):. 



