EXERCISE 96. TETANUS GROUP. 289 



Bacillus tetani Nicolaier. 



Explanatory. Discovered by Nicolaier, 1884. First cultivated 

 by Kitasato, 1889. Occurs in man and in animals suffering from 

 the disease, and is widely distributed in nature, especially in soil. 



Eeferences. Nicolaier, Deutsche Med. Wochenschrift, 1884; 

 Kitasato, Deutsche Med. Wochenschrift, 1889 ; A. 513 ; C. 302 ; PI. 2, 

 260 ; H. 320 ; K. & W. II, 566 ; L. & N. 332 ; Mig. 592 ; M. & R. 376 ; 

 McF. 389 ; P. 385. 



Morphological characters : 



Sketches. 



1. Form and arranoement: 

 a. Bouillon 



6. Agar . 



u. Gelatin 



d. Other media. . 



2. Size: 



3. Staininq powers: 



a. Aqueous gentian- violet. . 

 &. Loeffler's methylen-blue. 



c. Gram's stain 



d. Special stains 



4 :\IoTiLiTY : 



CI. Character of movement.., 



b. Flagrella stain 



5. Spores: 



Special characters: 



a. Capsules 



h. Involution forms , 



c. Deposits or vacuoles.. 



d. Pleomorphism 



