292 Physiological Chaeactees 



1. Kelation to temperature : 



optimum °C. ; limits to "C; 



thermal death-point °C. ; time of exposure minutes ; 



medium in which exposure is made 



2. Relation to free oxygen : 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc. ; 



4. Pigment production:, 



5. Gas production in sugar media: 



a. dextrose (1) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours percent. 



reaction in open arm 



gas formula, H: CO2: : 



h, lactose c. saccharose. 



6. Acid or ALKALI production: 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol peodoction 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



