318 MEDICAL BACTERIOLOGY 



i. Wash in distilled water. 



c. Tannic acid solution (10%) % to 1 minute. 



d. Counter-stain with a weak solution of eosin until sections are 



red. 



e. Wash in water until pink. 



/. Dehydrate in absolute alcohol. 



g. Clear in xylene and mount. 

 Bacterium diphtheriae. Loeffler's or better Weigert's method. 

 Bacillus typhosus. 



a. Loeffler's methylen blue or carbol-fuchsin 15 min. to 24 hrs. 



h. Wash slightly in distilled water. 



c. Place in 30% solution of tannic acid for 10 to 60 min. 



d. Dehydrate rapidly in alcohol. 



e. Clear in xylene. 

 /. Examine. 



g. Mount in balsam. 



Such sections examined under a low power will be found to con- 

 tain heavily stained masses, which under a high power prove to be 

 clumps of bacilli. Not infrequently the bacilli are difficult to detect 

 in tissue from typhoid cadavers. 

 Bacillus Salmonii (hog cholera). Loeffler's method. 

 Bacterium Welchii (gas bacillus) . Weigert's and Loeffler's methods. 

 Bacillus Feseri (symptomatic anthrax). Use Pfeiffer's stain : 



a. Dilute carbol-fuchsin % hour. 



h. Absolute alcohol slightly acidulated with acetic acid until 

 section is a reddish violet tint. 



c. Xylene and examine. 



d. Mount in balsam. 



Bacillus oedematis (malignant oedema). Pfeiffer's stain. 

 Streptothrix hovis (actinomyces). 



a. Ziehl's carbol-fuchsin, 10 minutes. 



i. Wash in distilled water. 



c. Picric acid (cons. ale. solution). 



d. Wash in distilled water. 



e. Wash in alcohol (50%). 



/. Dehydrate in absolute alcohol. 



g. Clear in xylene. 



li. Balsam. 



Tissue stained yellow, rays red. 



References. M. & W. 239-286 ; N. 537. 



