334 MEDICAL BACTERIOLOGY 



Bacterium tuberculosis. In case of miliary tuberculosis they 

 may be very rarely found in sufficient numbers to be detected by 

 staining methods, see sputum 101. 



Bacterium influenzae. Canon claims to have stained and culti- 

 vated this organ in blood, but this needs confirmation. 



Bacillus coli. This organism may be found in the blood. For 

 methods of isolation and identification see feces 103. 



Bacillus pestis. This germ occurs in the blood, in certain cases 

 at least. Considerable skill in detecting it is required— due to its 

 variable appearance. Broth tubes should be infected and animals 

 inoculated. 

 Bacillus Salmonii (hog cholera). 



a. Make agar plate and streak cultures from spleen of dead ani- 

 mal, and work iip the colonies as they appear. 

 6. Widal Reaction (for technique see below under B. typhosus). 

 Plasmodium malariae. 



a. Examination of fresh blood. A droplet of blood from finger, 

 or from lobe of ear, is placed on a glass slide, covered with 

 a cover-glass and then the cover-glass is ringed with vaselin. 

 Examination should be made with a ^ in. oil immersion. 

 h. Stained. Prepare films as directed above and stain with 

 methylen blue and eosin, or treat films with a very weak 

 acetic acid, 2 or 3 drops to 30 cc. of water ; to remove hemoglo- 

 bin, wash with water and stain with following solution for % 

 minute : 



Borax 5.0 parts. 



Methylen blue - 0.5 parts. 



Water 100 parts. 



Wash, dry and mount in balsam (Manson). 

 References, v. J. 45 ; Si. 100. See also texts under particular 

 organisms. 



Widal Reaction. Dried blood method. This method is espe- 

 cially valuable where patient is some distance from the laboratory. 

 Collect the blood as follows: "Wash with boiled water the part 

 from which the blood is to be obtained (lobe of ear, end of finger, or 

 toe in infant) . Prick deeply the skin with a needle, ' ' Remove two 

 or three large drops of blood on a clean glass slide, aluminum foil, 

 piece of isinglass or letter paper. AUmv the Hood to drtj. Then 

 place in an envelope and send to laboratory and test as follows : 



